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spp1  (R&D Systems)


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    R&D Systems spp1
    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of <t>SPP1,</t> SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af808/Mouse+Osteopontin%2FOPN+Antibody/pmc13078873-293-69-71
    Average 96 stars, based on 434 article reviews
    spp1 - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis"

    Article Title: Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI197556

    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Infection

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    Virus:

    Article Title: Axon guidance during mouse central nervous system regeneration is required for specific brain innervation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970; RRID: AB_300798 Goat polyclonal anti-VGLUT2 antibody Abcam Cat# ab178538; RRID: AB_3661773 Goat anti-CART antibody R&D Systems Cat# AF163; RRID: AB_2068569 Goat anti-parvalbumin antibody Abcam Cat# ab32895; RRID: AB_777105 Goat anti-osteopontin R&D Systems Cat# AF808; RRID: AB_2194992 Guinea polyclonal pig anti-RBPMS antibody Millipore Cat# ABN1376; RRID: AB_2687403 Mouse monoclonal anti-tubulin b3 (TUJ1) antibody Biolegend Cat# 801202;RRID: AB_10063408 Mouse monoclonal anti-PSD95 antibody Abcam Cat# ab2723 ; RRID: AB_303248 Mouse anti-Neurofilament H (NF-H), nonphosphorylated antibody (clone SMI-32) Biolegend Cat# 801701; RRID: AB_2564642 Rabbit polyclonal anti-VIP (Vasoactive Intestinal Peptide) antibody Immunostar Cat# 20077;RRID: AB_572270 Rabbit monoclonal anti-c-fos antibody Cell Signaling Technology Cat# 2250 ; RRID: AB_2247211 Rabbit anti-FOXP2 Abcam Cat# ab16046; RRID: AB_2107107 Rabbit polyclonal anti-melanopsin antibody Abcam Cat# ab19306; RRID: AB_444842 Rabbit polyclonal anti-Cre recombinase antibody Biolegend Cat# 908001; RRID: AB_2565079 Goat polyclonal anti-Robo1 antibody R&D Systems Cat# AF1749; RRID: AB_354969 Goat polyclonal anti-Robo2 antibody Dr Alain Chédotal (Institut de la Vision, Paris, France; MeLiS, University Claude Bernard Lyon 1, Hospices civils de Lyon, Lyon, France); Dominici et al.63 N/A Donkey anti-rabbit IgG (H+L) highly crossabsorbed secondary antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21206;RRID: AB_2535792 Donkey anti-rabbit IgG (H+L) highly crossabsorbed secondary antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573;RRID: AB_2536183 Donkey anti-chicken IgY (IgG) (H+L) antibody, Alexa Fluor 488 Jackson ImmunoResearch Labs Cat# 703-545-155; RRID: AB_2340375 Donkey anti-guinea pig IgG (H+L), Alexa Fluor 647 Jackson ImmunoResearch Labs Cat# 706-605-148; RRID: AB_2340476 Donkey anti-goat IgG (H+L), Alexa Fluor 488 Thermo Fisher Scientific Cat# A11055; RRID: AB_2534102 Donkey anti-mouse IgG (H+L), Alexa Fluor 647 Thermo Fisher Scientific Cat# A31571; RRID_162542 Donkey anti-mouse IgG (H+L), Alexa Fluor 568 Thermo Fisher Scientific Cat# A10037; RRID: AB_2534013 Bacterial and virus strains AAV2-Cre Belin et al.4 N/A AAV2-CNTF Belin et al.4 N/A AAV2-c-myc Belin et al.4 N/A AAV2-TVA-RG Produced using pAAV-DIO-TVA-V5-RG (see Recombinant DNA) N/A AAV2-pVIP-Cre This paper N/A (Continued on next page) Developmental Cell 59, 1–16.e1–e8, December 16, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primer ISH: Robo1 Forward; 5’-AGCAGTGGATGGCACTTTAA-3’ This paper N/A Primer ISH: Robo1 Reverse; 5’-GGAAAAGGTAAATGGCGTTA-3’ This paper N/A Primer ISH: Robo2 Forward; 5’-CTTTTCCCGAATCAACCTCA-3’ This paper N/A Primer ISH: Robo2 Reverse; 5’-GGGAGGTCATTCATATCATA-3’ This paper N/A Primer ISH: Sema6D Forward; 5’-TTCCTCCATGTGTGTCCTGT-3’ This paper N/A Primer ISH: Sema6D Reverse; 5’-GTTGGGTAAATGACTGGGTGATGT-3’ This paper N/A Primer ISH: Sfrp1 Forward; 5’-GGACCTGAGGCTGTGCCACA-3’ This paper N/A Primer ISH: Sfrp1 Reverse; 5’-TCTTCTTGGGGACAATCTTC-3’ This paper N/A Primer ISH: Sfrp2 Forward; 5’-GCCCAACCTGCTGGGCCACG-3’ This paper N/A Primer ISH: Sfrp2 Reverse; 5’-CGCCGTTCAGCTTGTAAATG-3’ This paper N/A Primer ISH: Slit1 Forward; 5’-CACTGGGTTGTTTAAGAAGC-3’ This paper N/A Primer ISH: Slit1 Reverse; 5’-CCTGGCCTTCCTCACACCTG-3’ This paper N/A Primer ISH: Slit2 Forward; 5’-CCTGCCAGCATGACTCCAAG-3’ This paper N/A Primer ISH: Slit2 Reverse; 5’-TCTATAGAGTTCCACGGCAA-3’ This paper N/A Primer ISH: VEGF-A Forward; 5’-GCAGCGACAAGGCAGACTA-3’ This paper N/A Primer ISH: VEGF-A Reverse; 5’-GCTAGCACTTCTCCCAGCTC-3’ This paper N/A Primer qPCR: Slit1 Forward; 5’-AGGTGCAAAAGGGCGAAT-3’ This paper N/A Primer qPCR: Slit1 Reverse; 5’-CGAGAGGGTACAGGCAGGT-3’ This paper N/A Primer qPCR: Slit2 Forward; 5’-CGGGGACAGCTGTGATAGAG-3’ This paper N/A Primer qPCR: Slit2 Reverse; 5’-CCAAGCGAGATACTTTCTTAGTTGT-3’ This paper N/A Primer qPCR: Slit3 Forward; 5’-gccacctcagtgagaacctc-3’ This paper N/A Primer qPCR: Slit3 Reverse; 5’-tgtccctcaaagcccaga-3’ This paper N/A Primer qPCR: Gapdh Forward; 5’-GCATGGCCTTCCGTGTTC-3’, This paper N/A Primer qPCR: Gapdh Reverse; 5’-TGTCATCATACTTGGCAGGTTTCT-3’ This paper N/A Recombinant DNA Plasmid ISH: Ephrin-B1 Dr Valérie Castellani (MeLiS, University Claude Bernard Lyon 1, Lyon, France) N/A Plasmid ISH: Netrin1 Dr Valérie Castellani (MeLiS, University Claude Bernard Lyon 1, Lyon, France); Nawabi et al.66 N/A (Continued on next page) Developmental Cell 59, 1–16.e1–e8, December 16, 2024 e3

    Produced:

    Article Title: Axon guidance during mouse central nervous system regeneration is required for specific brain innervation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970; RRID: AB_300798 Goat polyclonal anti-VGLUT2 antibody Abcam Cat# ab178538; RRID: AB_3661773 Goat anti-CART antibody R&D Systems Cat# AF163; RRID: AB_2068569 Goat anti-parvalbumin antibody Abcam Cat# ab32895; RRID: AB_777105 Goat anti-osteopontin R&D Systems Cat# AF808; RRID: AB_2194992 Guinea polyclonal pig anti-RBPMS antibody Millipore Cat# ABN1376; RRID: AB_2687403 Mouse monoclonal anti-tubulin b3 (TUJ1) antibody Biolegend Cat# 801202;RRID: AB_10063408 Mouse monoclonal anti-PSD95 antibody Abcam Cat# ab2723 ; RRID: AB_303248 Mouse anti-Neurofilament H (NF-H), nonphosphorylated antibody (clone SMI-32) Biolegend Cat# 801701; RRID: AB_2564642 Rabbit polyclonal anti-VIP (Vasoactive Intestinal Peptide) antibody Immunostar Cat# 20077;RRID: AB_572270 Rabbit monoclonal anti-c-fos antibody Cell Signaling Technology Cat# 2250 ; RRID: AB_2247211 Rabbit anti-FOXP2 Abcam Cat# ab16046; RRID: AB_2107107 Rabbit polyclonal anti-melanopsin antibody Abcam Cat# ab19306; RRID: AB_444842 Rabbit polyclonal anti-Cre recombinase antibody Biolegend Cat# 908001; RRID: AB_2565079 Goat polyclonal anti-Robo1 antibody R&D Systems Cat# AF1749; RRID: AB_354969 Goat polyclonal anti-Robo2 antibody Dr Alain Chédotal (Institut de la Vision, Paris, France; MeLiS, University Claude Bernard Lyon 1, Hospices civils de Lyon, Lyon, France); Dominici et al.63 N/A Donkey anti-rabbit IgG (H+L) highly crossabsorbed secondary antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21206;RRID: AB_2535792 Donkey anti-rabbit IgG (H+L) highly crossabsorbed secondary antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573;RRID: AB_2536183 Donkey anti-chicken IgY (IgG) (H+L) antibody, Alexa Fluor 488 Jackson ImmunoResearch Labs Cat# 703-545-155; RRID: AB_2340375 Donkey anti-guinea pig IgG (H+L), Alexa Fluor 647 Jackson ImmunoResearch Labs Cat# 706-605-148; RRID: AB_2340476 Donkey anti-goat IgG (H+L), Alexa Fluor 488 Thermo Fisher Scientific Cat# A11055; RRID: AB_2534102 Donkey anti-mouse IgG (H+L), Alexa Fluor 647 Thermo Fisher Scientific Cat# A31571; RRID_162542 Donkey anti-mouse IgG (H+L), Alexa Fluor 568 Thermo Fisher Scientific Cat# A10037; RRID: AB_2534013 Bacterial and virus strains AAV2-Cre Belin et al.4 N/A AAV2-CNTF Belin et al.4 N/A AAV2-c-myc Belin et al.4 N/A AAV2-TVA-RG Produced using pAAV-DIO-TVA-V5-RG (see Recombinant DNA) N/A AAV2-pVIP-Cre This paper N/A (Continued on next page) Developmental Cell 59, 1–16.e1–e8, December 16, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primer ISH: Robo1 Forward; 5’-AGCAGTGGATGGCACTTTAA-3’ This paper N/A Primer ISH: Robo1 Reverse; 5’-GGAAAAGGTAAATGGCGTTA-3’ This paper N/A Primer ISH: Robo2 Forward; 5’-CTTTTCCCGAATCAACCTCA-3’ This paper N/A Primer ISH: Robo2 Reverse; 5’-GGGAGGTCATTCATATCATA-3’ This paper N/A Primer ISH: Sema6D Forward; 5’-TTCCTCCATGTGTGTCCTGT-3’ This paper N/A Primer ISH: Sema6D Reverse; 5’-GTTGGGTAAATGACTGGGTGATGT-3’ This paper N/A Primer ISH: Sfrp1 Forward; 5’-GGACCTGAGGCTGTGCCACA-3’ This paper N/A Primer ISH: Sfrp1 Reverse; 5’-TCTTCTTGGGGACAATCTTC-3’ This paper N/A Primer ISH: Sfrp2 Forward; 5’-GCCCAACCTGCTGGGCCACG-3’ This paper N/A Primer ISH: Sfrp2 Reverse; 5’-CGCCGTTCAGCTTGTAAATG-3’ This paper N/A Primer ISH: Slit1 Forward; 5’-CACTGGGTTGTTTAAGAAGC-3’ This paper N/A Primer ISH: Slit1 Reverse; 5’-CCTGGCCTTCCTCACACCTG-3’ This paper N/A Primer ISH: Slit2 Forward; 5’-CCTGCCAGCATGACTCCAAG-3’ This paper N/A Primer ISH: Slit2 Reverse; 5’-TCTATAGAGTTCCACGGCAA-3’ This paper N/A Primer ISH: VEGF-A Forward; 5’-GCAGCGACAAGGCAGACTA-3’ This paper N/A Primer ISH: VEGF-A Reverse; 5’-GCTAGCACTTCTCCCAGCTC-3’ This paper N/A Primer qPCR: Slit1 Forward; 5’-AGGTGCAAAAGGGCGAAT-3’ This paper N/A Primer qPCR: Slit1 Reverse; 5’-CGAGAGGGTACAGGCAGGT-3’ This paper N/A Primer qPCR: Slit2 Forward; 5’-CGGGGACAGCTGTGATAGAG-3’ This paper N/A Primer qPCR: Slit2 Reverse; 5’-CCAAGCGAGATACTTTCTTAGTTGT-3’ This paper N/A Primer qPCR: Slit3 Forward; 5’-gccacctcagtgagaacctc-3’ This paper N/A Primer qPCR: Slit3 Reverse; 5’-tgtccctcaaagcccaga-3’ This paper N/A Primer qPCR: Gapdh Forward; 5’-GCATGGCCTTCCGTGTTC-3’, This paper N/A Primer qPCR: Gapdh Reverse; 5’-TGTCATCATACTTGGCAGGTTTCT-3’ This paper N/A Recombinant DNA Plasmid ISH: Ephrin-B1 Dr Valérie Castellani (MeLiS, University Claude Bernard Lyon 1, Lyon, France) N/A Plasmid ISH: Netrin1 Dr Valérie Castellani (MeLiS, University Claude Bernard Lyon 1, Lyon, France); Nawabi et al.66 N/A (Continued on next page) Developmental Cell 59, 1–16.e1–e8, December 16, 2024 e3

    Recombinant:

    Article Title: Axon guidance during mouse central nervous system regeneration is required for specific brain innervation.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken polyclonal anti-GFP antibody Abcam Cat# ab13970; RRID: AB_300798 Goat polyclonal anti-VGLUT2 antibody Abcam Cat# ab178538; RRID: AB_3661773 Goat anti-CART antibody R&D Systems Cat# AF163; RRID: AB_2068569 Goat anti-parvalbumin antibody Abcam Cat# ab32895; RRID: AB_777105 Goat anti-osteopontin R&D Systems Cat# AF808; RRID: AB_2194992 Guinea polyclonal pig anti-RBPMS antibody Millipore Cat# ABN1376; RRID: AB_2687403 Mouse monoclonal anti-tubulin b3 (TUJ1) antibody Biolegend Cat# 801202;RRID: AB_10063408 Mouse monoclonal anti-PSD95 antibody Abcam Cat# ab2723 ; RRID: AB_303248 Mouse anti-Neurofilament H (NF-H), nonphosphorylated antibody (clone SMI-32) Biolegend Cat# 801701; RRID: AB_2564642 Rabbit polyclonal anti-VIP (Vasoactive Intestinal Peptide) antibody Immunostar Cat# 20077;RRID: AB_572270 Rabbit monoclonal anti-c-fos antibody Cell Signaling Technology Cat# 2250 ; RRID: AB_2247211 Rabbit anti-FOXP2 Abcam Cat# ab16046; RRID: AB_2107107 Rabbit polyclonal anti-melanopsin antibody Abcam Cat# ab19306; RRID: AB_444842 Rabbit polyclonal anti-Cre recombinase antibody Biolegend Cat# 908001; RRID: AB_2565079 Goat polyclonal anti-Robo1 antibody R&D Systems Cat# AF1749; RRID: AB_354969 Goat polyclonal anti-Robo2 antibody Dr Alain Chédotal (Institut de la Vision, Paris, France; MeLiS, University Claude Bernard Lyon 1, Hospices civils de Lyon, Lyon, France); Dominici et al.63 N/A Donkey anti-rabbit IgG (H+L) highly crossabsorbed secondary antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21206;RRID: AB_2535792 Donkey anti-rabbit IgG (H+L) highly crossabsorbed secondary antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573;RRID: AB_2536183 Donkey anti-chicken IgY (IgG) (H+L) antibody, Alexa Fluor 488 Jackson ImmunoResearch Labs Cat# 703-545-155; RRID: AB_2340375 Donkey anti-guinea pig IgG (H+L), Alexa Fluor 647 Jackson ImmunoResearch Labs Cat# 706-605-148; RRID: AB_2340476 Donkey anti-goat IgG (H+L), Alexa Fluor 488 Thermo Fisher Scientific Cat# A11055; RRID: AB_2534102 Donkey anti-mouse IgG (H+L), Alexa Fluor 647 Thermo Fisher Scientific Cat# A31571; RRID_162542 Donkey anti-mouse IgG (H+L), Alexa Fluor 568 Thermo Fisher Scientific Cat# A10037; RRID: AB_2534013 Bacterial and virus strains AAV2-Cre Belin et al.4 N/A AAV2-CNTF Belin et al.4 N/A AAV2-c-myc Belin et al.4 N/A AAV2-TVA-RG Produced using pAAV-DIO-TVA-V5-RG (see Recombinant DNA) N/A AAV2-pVIP-Cre This paper N/A (Continued on next page) Developmental Cell 59, 1–16.e1–e8, December 16, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Primer ISH: Robo1 Forward; 5’-AGCAGTGGATGGCACTTTAA-3’ This paper N/A Primer ISH: Robo1 Reverse; 5’-GGAAAAGGTAAATGGCGTTA-3’ This paper N/A Primer ISH: Robo2 Forward; 5’-CTTTTCCCGAATCAACCTCA-3’ This paper N/A Primer ISH: Robo2 Reverse; 5’-GGGAGGTCATTCATATCATA-3’ This paper N/A Primer ISH: Sema6D Forward; 5’-TTCCTCCATGTGTGTCCTGT-3’ This paper N/A Primer ISH: Sema6D Reverse; 5’-GTTGGGTAAATGACTGGGTGATGT-3’ This paper N/A Primer ISH: Sfrp1 Forward; 5’-GGACCTGAGGCTGTGCCACA-3’ This paper N/A Primer ISH: Sfrp1 Reverse; 5’-TCTTCTTGGGGACAATCTTC-3’ This paper N/A Primer ISH: Sfrp2 Forward; 5’-GCCCAACCTGCTGGGCCACG-3’ This paper N/A Primer ISH: Sfrp2 Reverse; 5’-CGCCGTTCAGCTTGTAAATG-3’ This paper N/A Primer ISH: Slit1 Forward; 5’-CACTGGGTTGTTTAAGAAGC-3’ This paper N/A Primer ISH: Slit1 Reverse; 5’-CCTGGCCTTCCTCACACCTG-3’ This paper N/A Primer ISH: Slit2 Forward; 5’-CCTGCCAGCATGACTCCAAG-3’ This paper N/A Primer ISH: Slit2 Reverse; 5’-TCTATAGAGTTCCACGGCAA-3’ This paper N/A Primer ISH: VEGF-A Forward; 5’-GCAGCGACAAGGCAGACTA-3’ This paper N/A Primer ISH: VEGF-A Reverse; 5’-GCTAGCACTTCTCCCAGCTC-3’ This paper N/A Primer qPCR: Slit1 Forward; 5’-AGGTGCAAAAGGGCGAAT-3’ This paper N/A Primer qPCR: Slit1 Reverse; 5’-CGAGAGGGTACAGGCAGGT-3’ This paper N/A Primer qPCR: Slit2 Forward; 5’-CGGGGACAGCTGTGATAGAG-3’ This paper N/A Primer qPCR: Slit2 Reverse; 5’-CCAAGCGAGATACTTTCTTAGTTGT-3’ This paper N/A Primer qPCR: Slit3 Forward; 5’-gccacctcagtgagaacctc-3’ This paper N/A Primer qPCR: Slit3 Reverse; 5’-tgtccctcaaagcccaga-3’ This paper N/A Primer qPCR: Gapdh Forward; 5’-GCATGGCCTTCCGTGTTC-3’, This paper N/A Primer qPCR: Gapdh Reverse; 5’-TGTCATCATACTTGGCAGGTTTCT-3’ This paper N/A Recombinant DNA Plasmid ISH: Ephrin-B1 Dr Valérie Castellani (MeLiS, University Claude Bernard Lyon 1, Lyon, France) N/A Plasmid ISH: Netrin1 Dr Valérie Castellani (MeLiS, University Claude Bernard Lyon 1, Lyon, France); Nawabi et al.66 N/A (Continued on next page) Developmental Cell 59, 1–16.e1–e8, December 16, 2024 e3



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    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of <t>SPP1,</t> SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: The Journal of Clinical Investigation

    Article Title: Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis

    doi: 10.1172/JCI197556

    Figure Lengend Snippet: ( A ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice under chow diet. Expression was assessed by qRT-PCR ( n = 9–11). Mice were fed a chow diet and were sacrificed 8 weeks after infection with AAV. ( B ) Relative expression levels of key genes controlling lipogenesis, β-oxidation, gluconeogenesis, and fibrosis in livers of mice after 10 weeks on HFD. Expression was assessed by qRT-PCR ( n = 7). Mice were fed an HFD for 2 weeks before infection with AAV and were sacrificed 8 weeks later. ( C ) Protein levels of SREBP-1c, SCD1, and CHAC1 in livers of mice under HFD. ( D ) Relative expression levels of Chac1 -regulated genes in primary hepatocytes under 100 μM free fatty acid (FA) treatment. Expression was assessed by qRT-PCR ( n = 3). Adenovirus (Adv): 400 μL/mL medium. Cells were harvested 3 days postinfection. ( E ) Protein levels of SPP1, SCD1, and CHAC1 in primary hepatocytes under 100 μM free FA treatment. Primary hepatocytes were infected with Adv overexpressing GFP or Chac1 . Adv: 400 μL/mL medium. Cells were harvested 3 days postinfection. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Immunoblotting was achieved using the indicated antibodies: phospho-IR Y1152/Y1153 (3024; Cell Signaling Technology [CST]), IR (sc-711), phospho-AKT S473 (4060; CST), phospho-AKT T308 (4056; CST), AKT (4685; CST), phospho-IR T1150 (NBP2-60777; Novus Biologicals), phospho-IRS1 S1097 (2385; CST), phospho-JNK (4668; CST), JNK (9252; CST), CHAC1 (ab76386, Abcam), Vinculin (MAB3574, Sigma), FASN (ab22759, Abcam), SCD1 (2438, CST), total OXPHOS (ab110413, Abcam), NOTCH1 (3608, CST), NOTCH2 (5732, CST), HES1 (sc-166410, Santa Cruz Biotechnology), SPP1 (AF808, R&D Systems), and β-tubulin (2146; CST).

    Techniques: Expressing, Quantitative RT-PCR, Infection